Water Hyacinth's Extent and Its Implication on Water Quality in Lake Victoria, Uganda.

Water hyacinth (Eichhornia crassipes) degrades and obstructs the integrity of freshwater ecosystems. However, little attention has been paid to monitoring water hyacinth's spatial extent, its determinants, and its effects on water quality in Lake Victoria, Uganda. The specific objectives of this paper are to (i) assess the spatial extent and distribution of water hyacinth; (ii) examine the determinants of water hyacinth distribution, and (iii) assess its impact on water quality. High-resolution satellite images (2016-2019) were obtained and used to monitor the spatial extent of the water hyacinth, a household survey was conducted to examine the determinants of the water hyacinth's extent and patterns while water samples were drawn and analysed for physicochemical properties. Results show that the coverage and distribution of water hyacinth varied over space and time. Water hyacinth coverage primarily increased with a decrease in water surface area. The perceived factors that triggered the water hyacinth spread included the morphology of the Bay, effluent discharge, strong winds, speed of water current, water-level changes, ferry navigation, and construction activities at the shore. Water parameters significantly impacted by hyacinth were pH, TP, BOD, COD, DO, turbidity, and transparency. This study recommends the strict development and implementation of integrated weed control measures, catchment management plans, and point and nonpoint pollution source control.

Carbon emission fluctuations of Chinese inter-regional interaction: a network multi-hub diffusion perspective.

The "double-carbon" policy is a new opportunity for the transformation of China's production sector. With steady economic growth, each province has proposed specific policies aimed at cleaner production. However, the interactions between regions and the complex linkages between industries have hindered the implementation of the "double-carbon" policy. In order to address this issue, we introduced a complex network framework with multiple industries at a national level. The framework aimed to clarify whether there is fluctuation diffusion in China's multi-province multi-industry carbon emission system, to identify key industries and regions, and to answer the question of "who" is the most effective in governance. The results showed that the fluctuations of industrial carbon emissions had a cross-regional diffusion effect in China indeed. The diffusion capacity of industry fluctuation depends on whether the industry is located at a "hub" position in the network. Hub industries with strong capacity can spread the carbon emission fluctuation of themselves and upstream or downstream industries to the whole country through regional interactions. This characteristic of the hub industry should be taken into account in governance to maximize the effectiveness of emission reduction. Shandong and Inner Mongolia, as important provinces for the production of intermediate products and energy chemicals in China, had a greater role to play in global carbon supply push from their hub industries than in the demand pull. The pulling capacity of Beijing-Tianjin-Hebei region to the national carbon demand side was greater than that of Yangtze River Delta and Pearl River Delta. These findings might have implications for environmental and economic policymaking, particularly with regard to cross-provincial coordinated systemic solutions and policy anchors for synergy with industries.

Map of Evidence: Interventions to Reduce Exposure to Household Air Pollution

This evidence map was developed to identify, summarize, and communicate to key stakeholders the findings of evidence on interventions to reduce exposure to household air pollution (HAP). The map synthesizes evidence from 48 literature reviews published on technological and sociocultural interventions and others (potentialities of public policies like incentives) to address health, environmental and economic impacts associated to HAP. Furthermore, this map seeks to inform decision-making processes on strategic interventions and most effective policies. Main results: - Most of the existing HAP interventions to address indoor air quality have been focused on evaluating improved stove interventions that still use solid fuels (n = 39). It has been identified that Interventions with clean fuels (LPG, electricity, and others) are the most effective in impacting the health outcomes and reducing household air pollutants. - Only six reviews analyzed or reported outcomes of sociocultural interventions (health education) aimed at reducing air pollution in homes; some showed positive impacts on health. Three reviews reported the effects of changes in people's behaviors when cooking food, which have improved indoor air quality. All authors agree on the importance of changing behavior and community practices as an essential element for the sustainability of improved kitchen interventions. - Four reviews evaluated at the synergy between policies to reduce household air pollution and outdoor pollution, as well as other environmental health concerns. These reviews allow the conclusion that synergies between policies are much more effective at reducing air pollution and its impacts on the health of communities.

The use of BCYE medium for the detection of Legionella in environmental water samples: an appropriate update to ISO 11731:2017 standard?

We evaluated the diagnostic performances of 2 media (BCYE, MWY) on 951 Legionella-positive hospital water samples. MWY allowed detecting Legionella in 89.2% of samples, but in 10.8% (103/951) Legionella was found on BCYE plates only. In samples where Legionella was isolated with other microorganisms (663/951), MWY was essential to detect the majority of positive samples (349/663, 52.6%), as fewer plates resulted unreadable; however, in those containing Legionella only, a higher frequency of positive samples was recorded with BCYE (94.8%, 273/288) compared to MWY (85.1%, 245/288). Considering the 484 concordant positive samples, overall Legionella counts were significantly higher on BCYE (P = 0.0029), with 47% of samples showing higher counts on BCYE compared to MWY plates. Furthermore, discordant samples (positive on only one medium) showed different relative proportions between Legionella pneumophila and non-pneumophila, the latter being found more frequently on BCYE only (P = 0.0296).Our findings confirm the appropriateness of the ISO 11731:2017 update.

A quantitative evaluation of aerosol generation during supraglottic airway insertion and removal.

Many guidelines consider supraglottic airway use to be an aerosol-generating procedure. This status requires increased levels of personal protective equipment, fallow time between cases and results in reduced operating theatre efficiency. Aerosol generation has never been quantitated during supraglottic airway use. To address this evidence gap, we conducted real-time aerosol monitoring (0.3-10-µm diameter) in ultraclean operating theatres during supraglottic airway insertion and removal. This showed very low background particle concentrations (median (IQR [range]) 1.6 (0-3.1 [0-4.0]) particles.l-1 ) against which the patient's tidal breathing produced a higher concentration of aerosol (4.0 (1.3-11.0 [0-44]) particles.l-1 , p = 0.048). The average aerosol concentration detected during supraglottic airway insertion (1.3 (1.0-4.2 [0-6.2]) particles.l-1 , n = 11), and removal (2.1 (0-17.5 [0-26.2]) particles.l-1 , n = 12) was no different to tidal breathing (p = 0.31 and p = 0.84, respectively). Comparison of supraglottic airway insertion and removal with a volitional cough (104 (66-169 [33-326]), n = 27), demonstrated that supraglottic airway insertion/removal sequences produced <4% of the aerosol compared with a single cough (p < 0.001). A transient aerosol increase was recorded during one complicated supraglottic airway insertion (which initially failed to provide a patent airway). Detailed analysis of this event showed an atypical particle size distribution and we subsequently identified multiple sources of non-respiratory aerosols that may be produced during airway management and can be considered as artefacts. These findings demonstrate supraglottic airway insertion/removal generates no more bio-aerosol than breathing and far less than a cough. This should inform the design of infection prevention strategies for anaesthetists and operating theatre staff caring for patients managed with supraglottic airways.

Toxicological assessment method for evaluating the occupational risk of dynamic olfactometry assessors.

The paper aims to propose a new method to evaluate the occupational exposure risk for examiners involved in dynamic olfactometry. Indeed, examiners are possibly exposed to hazardous pollutants potentially present in odorous samples. A standardized method to evaluate the examiners' occupational safety is not yet available and the existing models present some critical aspect if applied to real odorous samples (no uniform reference concentrations applied and presence of compounds for which no toxicity threshold is available). A deepening of assessment procedure to evaluate the occupation exposure risk for olfactometric assessors is necessary. This paper proposes a standardized approach for risk assessment in dynamic olfactometry. The proposed approach allows the quantification synthetic and conservative risk indices. In this model, the use of the hazard index for the odorous mixture was proposed to assess the non-carcinogenic risk; the calculation of the inhalation risk was applied to estimate the carcinogenic risk. Different databases can be used to retrieve proper occupational exposure limits, according to the proposed hierarchical basis. These implementations allow obtaining the complete characterization of real samples which can be used to calculate the minimum dilution factor for protecting the panellists' health.

Quantitative Approach Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-ToF) Mass Spectrometry.

Quantitation using mass spectrometry (MS) is a routine approach for multiple analytes, including small molecules and peptides. Electrospray-based MS platforms are typically employed, as they provide highly reproducible outputs for batch processing of multiple samples. Quantitation using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF) mass spectrometry, while less commonly adopted, offers the ability to monitor analytes at significantly higher throughput and lower cost compared with ESI MS. Achieving accurate quantitation using this approach requires the development of appropriate sample preparation, spiking of appropriate internal standards, and acquisition to minimize spot-to-spot variability. Here we describe the preparation of samples for accurate quantitation using MALDI-ToF MS. The methodology presented shows the ability to quantitate perfluorooctanesulfonic acid (PFOS) from contaminated water.

Evidence of air quality data misreporting in China: An impulse indicator saturation model comparison of local government-reported and U.S. embassy-reported PM2.5 concentrations (2015-2017).

This paper analyzes hourly PM2.5 measurements from government-controlled and U.S. embassy-controlled monitoring stations in five Chinese cities between January 2015 and June 2017. We compare the two datasets with an impulse indicator saturation technique that identifies hours when the relation between Chinese and U.S. reported data diverges in a statistically significant fashion. These temporary divergences, or impulses, are 1) More frequent than expected by random chance; 2) More positive than expected by random chance; and 3) More likely to occur during hours when air pollution concentrations are high. In other words, relative to U.S.-controlled monitoring stations, government-controlled stations systematically under-report pollution levels when local air quality is poor. These results contrast with the findings of other recent studies, which argue that Chinese air quality data misreporting ended after a series of policy reforms beginning in 2012. Our findings provide evidence that local government misreporting did not end after 2012, but instead continued in a different manner. These results suggest that Chinese air quality data, while still useful, should not be taken entirely at face value.

How accurately can we assess zoonotic risk?

Identifying the animal reservoirs from which zoonotic viruses will likely emerge is central to understanding the determinants of disease emergence. Accordingly, there has been an increase in studies attempting zoonotic "risk assessment." Herein, we demonstrate that the virological data on which these analyses are conducted are incomplete, biased, and rapidly changing with ongoing virus discovery. Together, these shortcomings suggest that attempts to assess zoonotic risk using available virological data are likely to be inaccurate and largely only identify those host taxa that have been studied most extensively. We suggest that virus surveillance at the human-animal interface may be more productive.

Utilization of a NIST SRM: a case study for per- and polyfluoroalkyl substances in NIST SRM 1957 organic contaminants in non-fortified human serum.

The National Institute of Standards and Technology (NIST) generates and maintains thousands of Standard Reference Materials (SRMs) to serve commerce worldwide. Many SRMs contain metrologically traceable mass fractions of known organic chemicals and are commercially available to aid the analytical chemistry community. One such material, NIST SRM 1957 Organic Contaminants in Non-Fortified Human Serum, was one of the first materials issued by NIST with measurements for per- and polyfluoroalkyl substances (PFAS) listed on the Certificate of Analysis and was commercially available in 2009. Since the release of SRM 1957, nearly 400 units have been sold to date, and over 50 publications related to PFAS measurements have included this material for multiple analytical purposes, such as a quality control material, for interlaboratory comparison, as an in-house comparison tool, for inter- and intra-day measurement accuracy, as an indicator of isomeric patterns of PFAS, and for other uses. This perspective details the ways SRM 1957 is utilized by the analytical community and how data have been reported in the literature. A discussion on accurately comparing SRM data to generated data is included. Furthermore, we conducted an in-depth investigation around additional applications for NIST SRMs, such as a matrix-matched reference material, and for the identification of targeted compounds during high-resolution mass spectrometry data collection. Ultimately, this manuscript illustratively describes the ways to utilize a NIST SRMs for chemicals of emerging concern.

What is necessary for next-generation atmospheric environmental standards? Recent research trends for PM2.5 -bound polycyclic aromatic hydrocarbons and their derivatives.

The air pollution associated with PM2.5 kills 7 million people every year in the world, especially threatening the health of children in developing countries. However, the current air quality standards depend mainly on particle size. PM2.5 contains many carcinogenic/mutagenic polycyclic aromatic hydrocarbons (PAHs) and their derivatives such as nitropolycyclic aromatic hydrocarbons and oxygenated PAHs. Among them, environmental standards and guidelines have been set for benzo[a]pyrene by few countries and international organizations. Recent research reports showed that these pollutants are linked to diseases other than lungs, and new methods have been developed for determining trace levels of not only PAHs but also their derivatives. It is time to think about the next-generation environmental standards. This article aims to (a) describe recent studies on the health effects of PAHs and their derivatives other than cancer, (b) describe new analytical methods for PAH derivatives, and (c) discuss the targets for the next-generation standards.

Can we manage fisheries with the inherent uncertainty from eDNA?

Environmental (e)DNA, as a general approach in aquatic systems, seeks to connect the presence of species' genetic material in the water and hence to infer the species' physical presence. However, fisheries managers face making decisions with risk and uncertainty when eDNA indicates a fish is present but traditional methods fail to capture the fish. In comparison with traditional methods such as nets, electrofishing and piscicides, eDNA approaches have more sources of underlying error that could give rise to false positives. This has resulted in some managers to question whether eDNA can be used to make management decisions because there is no fish in hand. As a relatively new approach, the methods and techniques have quickly evolved to improve confidence in eDNA. By evaluating an eDNA based research programmes through the pattern of the eDNA signal, assay design, experimental design, quality assurance and quality control checks, data analyses and concurrent search for fish using traditional gears, the evidence for fish presence can be evaluated to build confidence in the eDNA approach. The benefits for fisheries management from adopting an eDNA approach are numerous but include cost effectiveness, broader geographic coverage of habitat occupancy, early detection of invasive species, non-lethal stock assessments, exploration of previously inaccessible aquatic environments and discovery of new species hidden beneath the water's surface. At a time when global freshwater and marine fisheries are facing growing threats from over-harvest, pollution and climate change, we anticipate that growing confidence in eDNA will overcome the inherent uncertainty of not having a fish in hand and will empower the informed management actions necessary to protect and restore our fisheries.

Segregation of respirable dust for chemical and toxicological analyses.

Respirable dust can pass beyond ciliated airways of the respiratory tract and influence adverse health effects. Health effects can be studied using samples generated from bulk dust segregation. Because previous segregation methods diverge from size-selection criteria of the international convention for respirable particles (ICRP), a method was developed to approximate the ICRP. The method was compared to an ideal sampler by measuring the sample collection bias. The comparison shows that the uncertainty due to the bias was 0.10 based on European Standard EN13205:2014 criteria, which indicates that the segregator effectively follows the ICRP. Respirable particle size distributions were confirmed by an aerodynamic particle sizer and by computer-controlled scanning electron microscopy. Consequently, a systematic way to generate respirable powders for health effects studies and chemical analyses was developed.

Testing the validity of a proposed dermal cancer slope factor for Benzo[a]pyrene.

In 2014, the United States Environmental Protection Agency (EPA) proposed a Dermal Slope Factor (DSF) for benzo[a]pyrene (BaP) of 0.006 (µg/day)-1 (USEPA 2014a). It would make cancer risk estimates associated with soil contact 100 times greater than those from soil ingestion and would predict that a large fraction of skin Basal Cell Carcinomas (BCCs) and Squamous Cell Carcinomas (SCCs) worldwide are caused by low level dermal exposures to PAHs, such as BaP. This is not logical given that sunlight (ultraviolet radiation (UV)) exposure is the generally recognized cause of BCCs and SCCs. This paper critically evaluates the proposed DSF. First, a reality check is performed using EPA standard risk assessment methods and comparing the results to actual BCC and SCC rates in the U.S. population. Then, the biological plausibility of the mechanism by which PAHs might cause human skin cancer is evaluated by exploring the generally recognized etiology of human skin cancer and comparing the genetic mutation signatures of rodent skin tumors caused by PAH exposures to those of human skin cancers. It is concluded that scientific flaws resulted in a proposed DSF value that greatly overestimates the skin cancer risk for humans dermally exposed to BaP in soil.

Industrial Applications of Dinoflagellate Phycotoxins Based on Their Modes of Action: A Review.

Dinoflagellates are an important group of phytoplanktons, characterized by two dissimilar flagella and distinctive features of both plants and animals. Dinoflagellate-generated harmful algal blooms (HABs) and associated damage frequently occur in coastal areas, which are concomitant with increasing eutrophication and climate change derived from anthropogenic waste and atmospheric carbon dioxide, respectively. The severe damage and harmful effects of dinoflagellate phycotoxins in the fishing industry have been recognized over the past few decades, and the management and monitoring of HABs have attracted much attention, leaving aside the industrial application of their valuable toxins. Specific modes of action of the organisms' toxins can effectively be utilized for producing beneficial materials, such as Botox and other therapeutic agents. This review aims to explore the potential industrial applications of marine dinoflagellate phycotoxins; furthermore, this review focuses on their modes of action and summarizes the available knowledge on them.

Effect of Inter-Observer Variation on the Association between Contamination Hazards and the Microbiological Quality of Water Sources: A Longitudinal Study.

Sanitary risk inspection protocols are often used to identify contamination hazards at water sources; however, different observers sometimes struggle to record hazards consistently. This study aimed to assess the effect of inter-observer variation in hazard observations on the strength of relationships between observed hazards and the bacterial contamination of water sources, particularly relationships with animal-related hazards. In a longitudinal study, five surveyors independently recorded hazards at 93 water sources used by 234 households in Siaya County, Kenya, in both wet and dry seasons. One surveyor collected samples from sources for subsequent Escherichia coli and intestinal enterococci testing. The relationship between each surveyor's hazard observations and high bacterial contamination was examined using logistic regression. After controlling for water source type and preceding rainfall; percentage scores for animal-related hazards were significantly related to high contamination with enterococci and E. coli for one surveyor (odds ratio 1.02; 95% confidence intervals 1.00-1.03 for both parameters), but not for the remaining four surveyors. The relationship between observed contamination hazards and the microbiological contamination of water sources is sensitive to variation in hazard recording between surveyors. Sanitary risk protocols should be designed to enable robust and consistent observation of hazards.

Personal-Level Protective Actions Against Particulate Matter Air Pollution Exposure: A Scientific Statement From the American Heart Association.

Since the publication of the last American Heart Association scientific statement on air pollution and cardiovascular disease in 2010, unequivocal evidence of the causal role of fine particulate matter air pollution (PM2.5, or particulate matter ≤2.5 µm in diameter) in cardiovascular disease has emerged. There is a compelling case to provide the public with practical personalized approaches to reduce the health effects of PM2.5. Such interventions would be applicable not only to individuals in heavily polluted countries, high-risk or susceptible individuals living in cleaner environments, and microenvironments with higher pollution exposures, but also to those traveling to locations with high levels of PM2.5. The overarching motivation for this document is to summarize the current evidence supporting personal-level strategies to prevent the adverse cardiovascular effects of PM2.5, guide the use of the most proven/viable approaches, obviate the use of ineffective measures, and avoid unwarranted interventions. The significance of this statement relates not only to the global importance of PM2.5, but also to its focus on the most tested interventions and viable approaches directed at particulate matter air pollution. The writing group sought to provide expert consensus opinions on personal-level measures recognizing the current uncertainty and limited evidence base for many interventions. In doing so, the writing group acknowledges that its intent is to assist other agencies charged with protecting public health, without minimizing the personal choice considerations of an individual who may decide to use these interventions in the face of ongoing air pollution exposure.